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Zyagen provides researchers with access to
high quality tissue lysates (total protein) of rat and mouse tissues that might
difficult to obtain because of size, anatomical complexity, or high protease
activity.
Tissues are dissected and homogenized in RIPA
lysis buffer supplemented with a cocktail of 7 protease inhibitors to
minimize proteolysis.
The extracted protein is precisely quantified,
and packed in 1.5-ml tubes at a concentration of 5 mg/ml. Protein is stable for
one year if stored at -80oC.
The quality of protein as indicated by the absence of smear (no degradation) and
sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with
Coomassie blue staining. The integrity of protein is tested by
immunostaining using specific beta-actin antibody.
The extracted protein is ready for immediate
use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing
gels and SDS-capillary electrophoresis.
Custom Services: If you are interested in tissue lysate
that is not listed below or required tissue lysate to be specifically prepared
to meet your application, our custom service scientists can make it for you.
Click
here
for more information.
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