Zyagen offers high quality ready to use total
protein of freshly harvested mouse and rat embryos at different stages of
pregnancy. The
pregnancy is precisely determined by observation of vaginal plug. Plug date is considered
day one of gestation.
Tissues are freshly dissected and homogenized
in RIPA buffer supplemented with a cocktail of 7 protease inhibitors to minimize
proteolysis. The extracted proteins are precisely quantified, and packed in
1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if
stored properly.
The
quality of protein as indicated by the absence of smear (no degradation) and
sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with
Coomassie blue staining. The integrity of protein is tested by
immunoblotting using specific beta-actin antibody.
The extracted protein is ready for immediate
use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing
gels and SDS-capillary electrophoresis.
Custom Services: Zyagen also offers
custom service to prepare ready to use total protein of embryonic tissue of any mouse
and rat strain. Contact customer services/technical support for more
information.
