Zyagen offers high quality ready to use total protein of
freshly harvested mouse and rat brain tissues. Tissues are freshly dissected and
homogenized in lysis buffer supplemented with a cocktail of protease inhibitors
to minimize proteolysis. The extracted proteins are precisely quantified, and
packed in 1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one
year if stored properly.
The
quality of protein as indicated by the absence of smear (no degradation) and
sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with
Coomassie blue staining. The integrity of protein is tested by
immunoblotting using specific beta-actin antibody.
The extracted protein is ready for immediate
use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing
gels and SDS-capillary electrophoresis.
Custom Services: Zyagen also offers
custom service to prepare ready to use total protein of brain tissue of any mouse
and rat strain. Contact customer services/technical support for more
information.
