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Zyagen offers high quality ready to use total protein of freshly harvested mouse and rat mammary glands at different stages of pregnancy, lactation, and involution. Pregnancy is precisely determined by observation of vaginal plug. Plug date is considered  day one of gestation. 

Tissues are freshly dissected and homogenized in RIPA buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis. The extracted proteins are precisely quantified, and packed in 1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if stored properly.

The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.

The extracted protein is ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis.

Custom Services: Zyagen also offers custom service to prepare ready to use total protein of mammary gland tissues of any mouse and rat strain. Contact customer services/technical support for more information.