Zyagen offers high quality ready to use total protein
of freshly harvested mouse and rat placenta at different stages of pregnancy. The
pregnancy is precisely determined by observation of vaginal plug. Plug date is considered
day one of gestation.
Tissues are freshly dissected and homogenized
in RIPA buffer supplemented with a cocktail of 7 protease inhibitors to minimize
proteolysis. The extracted proteins are precisely quantified, and packed in
1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if
stored properly.
The
quality of protein as indicated by the absence of smear (no degradation) and
sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with
Coomassie blue staining. The integrity of protein is tested by
immunoblotting using specific beta-actin antibody.
The extracted protein is ready for immediate
use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing
gels and SDS-capillary electrophoresis.
Custom Services: Zyagen also offers
custom service to prepare ready to use total protein of placental tissues of any mouse
and rat strain. Contact customer services/technical support for more
information.
Mouse Pregnancy-staged Placenta
Total Protein Ordering Information
Embryonic Stage
Mouse Total Protein
Quantity
Price
Placenta-E10
MT-413-10
0.5mg
$110
Placenta-E11
MT-413-11
0.5mg
$110
Placenta-E12
MT-413-12
0.5mg
$110
Placenta-E13
MT-413-13
0.5mg
$110
Placenta-E14
MT-413-14
0.5mg
$110
Placenta-E15
MT-413-15
0.5mg
$110
Placenta-E16
MT-413-16
0.5mg
$110
Placenta-E17
MT-413-17
0.5mg
$110
Placenta-E18
MT-413-18
0.5mg
$110
Set of any 5 placenta Total Protein, 0.1mg each
MT-413-005
5x0.1mg
$350
Set of all above 9 placenta Total Protein,0.1mg each
MT-413-009
9x0.1mg
$549
Rat Pregnancy-staged Placenta Total Protein
Ordering Information
Embryonic Stage
Rat Total Protein
Quantity
Price
Placenta-E12
RT-413-12
0.5mg
$110
Placenta-E13
RT-413-13
0.5mg
$110
Placenta-E14
RT-413-14
0.5mg
$110
Placenta-E15
RT-413-15
0.5mg
$110
Placenta-E16
RT-413-16
0.5mg
$110
Placenta-E17
RT-413-17
0.5mg
$110
Placenta-E18
RT-413-18
0.5mg
$110
Placenta-E19
RT-413-19
0.5mg
$110
Placenta-E20
RT-413-20
0.5mg
$110
Set of any 5 placenta Total Protein, 0.1mg each
RT-413-005
5x0.1mg
$350
Set of all above 9 placenta Total Protein,0.1mg each
