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Biochemicals, Kits and Reagents

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Zyagen provides researchers with access to high quality tissue lysates (total protein) of rat and mouse tissues that might difficult to obtain because of size, anatomical complexity, or high protease activity.

Tissues are dissected and homogenized in RIPA lysis buffer supplemented with a cocktail of 7 protease inhibitors to minimize proteolysis.

The extracted protein is precisely quantified, and packed in 1.5-ml tubes at a concentration of 5 mg/ml. Protein is stable for one year if stored at -80^oC.

The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunostaining using specific beta-actin antibody.

The extracted protein is ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis.

Custom Services: If you are interested in tissue lysate that is not listed below or required tissue lysate to be specifically prepared to meet your application, our custom service scientists can make it for you. Click here for more information.