Zyagen offers high quality ready to use total protein of
human adult tissues. Tissues are freshly dissected and homogenized
in RIPA buffer supplemented with a cocktail of 7 protease inhibitors to minimize
proteolysis. The extracted proteins are precisely quantified, and packed in
1.5-ml tubes at a concentration of 5mg/ml. Protein is stable for one year if
stored properly.
The
quality of protein as indicated by the absence of smear (no degradation) and
sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with
Coomassie blue staining. The integrity of protein is tested by
immunoblotting using specific beta-actin antibody.
The extracted protein is ready for immediate
use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing
gels and SDS-capillary electrophoresis.
