Zyagen offers high quality pure intact total
RNA of freshly harvested human tissues. RNA samples are extracted using
a modified
guanidine thiocyanate-phenol/chloroform method, treated with RNase-free DNase to remove residual DNA,
precisely quantified,
and stored at -80oC. The integrity of each RNA
sample as
indicated by intact 28S and 18S ribosomal RNA is verified by denatured agarose
gel electrophoresis. The purity of RNA is assessed by spectrophotometer
(A260/A280 >1.9).
RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease
assay, RT-PCR analysis, rapid
amplification of cDNA ends (RACE) and purification of mRNA for library construction.
Total RNA is provided in RNase-free water, 1mM sodium
citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml and shipped on dry ice.
