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Zyagen offers high quality pure intact total RNA of freshly harvested mouse and rat whole embryos at different stages of pregnancy. The pregnancy is precisely determined by observation of vaginal plug. Plug date is considered  day one of gestation. 

RNA samples are extracted using a modified guanidine thiocyanate-phenol/chloroform method, treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80^oC. The integrity of each RNA sample as indicated by intact 28S and 18S ribosomal RNA  is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280 >1.9).

RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR analysis, rapid amplification of cDNA ends (RACE) and purification of mRNA for library construction.

Total RNA is provided in RNase-free water, 1mM sodium citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml and shipped on dry ice.

Custom RNA Isolation Services: Zyagen also offers custom service to prepare ready to use Total RNA and mRNA of embryonic tissue of any mouse and rat strain. Contact customer services/technical support for more information.