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Zyagen offers high quality pure intact total
RNA of
almost all rat and mouse tissues. RNA is
freshly harvested from mouse and rat tissues including adult
tissues,
developmental (age related) tissues, and pregnancy-staged embryo, placenta, uterus, and
mammary gland.
RNA samples are extracted using a modified
guanidine thiocyanate-phenol/chloroform method, treated with RNase-free DNase to remove residual DNA,
precisely quantified,
and stored at -80oC. The integrity of each RNA
sample as
indicated by intact 28S and 18S ribosomal RNA is verified by denatured agarose
gel electrophoresis. The purity of RNA is assessed by spectrophotometer
(A260/A280 >1.9).
RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease
assay, RT-PCR analysis, rapid
amplification of cDNA ends (RACE) and purification of mRNA for library construction.
Total RNA is provided in RNase-free water, 1mM sodium
citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml and shipped on dry ice.
Custom RNA Isolation Services: Zyagen also offers
custom service to prepare ready to use Total RNA and mRNA of any mouse
and rat strain. Contact customer services/technical support for more
information.
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