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C57 Mouse Mammary Tissue Lysates

Zyagen provides high quality tissue lysates (total protein) of mouse C57BL/6 mammary gland at different stages, pregnancy, lactation, and invoultion.

 Mammary gland tissues are collected and homogenized in protein lysis buffer supplemented with a cocktail of 7 mammlian protease inhibitors to minimize proteolysis.

The extracted protein is precisely quantified, and packed in 1.5-ml tubes at a concentration of 5 mg/ml. Protein is stable for one year if stored at -80oC.

The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunostaining using specific beta-actin antibody.

The extracted protein is provided in vials at a concentration of 5mg/ml and and ready for immediate use in reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, and protein-protein interaction. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use. Protein can also be provided in denatured SDS Sample buffer for Western blotting.

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