Your Innovative Source of Life Science Products and Services

SD Rat Embryo Tissue Lysates

Zyagen provides high quality tissue lysates (total protein) of Sprague Dawley rat whole embryo at different stages of pregnancy (E12 to E20). The pregnancy is precisely determined by observation of vaginal plug. Plug date is considered  day one of gestation.

Embryos are collected and homogenized in protein lysis buffer supplemented with a cocktail of 7 mammlian protease inhibitors to minimize proteolysis.

The extracted protein is precisely quantified, and packed in 1.5-ml tubes at a concentration of 5 mg/ml. Protein is stable for one year if stored at -80oC.

The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by immunostaining using specific beta-actin antibody.

The extracted protein is provided in vials at a concentration of 5mg/ml and ready for immediate use in the Lysis Buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins. Proteins extracted can be readily used in many applications, including reporter assays, protein assays, immunoassays, SDS-PAGE, protein purification, and many other applications. Protein can also be provided in denatured SDS Sample buffer for only Western Blotting use.

Showing all 11 results

X