AIM Antibody: Apoptosis inhibitor of macrophages (AIM) is a member of the scavenger receptor cysteine-rich domain superfamily (SRCR-SF) initially identified as an inducible cell surface ligand of CD5. It was shown that AIM functions in the thymus as the inducer of resistance to apoptosis within CD4+/CD8+ thymocytes and as the supporter of the viability of these cells before thymic selection. AIM was also shown to support macrophage survival and enhance their phagocytic function. More recent experiments using recombinant AIM significantly inhibited apoptosis of NKT and T cells obtained from C. parvum-stimulated livers in vitro, suggesting that AIM functions to induce resistance to apoptosis in these cells and supports host defense against inflammation during infection.
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Description
Western blot analysis of AIM in human lymph node tissue lysate with AIM antibody at (A) 1, (B) 2 and (C) 4 ?g/mL.
Immunohistochemistry of AIM in human lymph node tissue with AIM antibody at 2 ?g/mL.
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Specifications
Additional Names
AIM Antibody: Apoptosis inhibitor of macrophages, API6, SP alpha, CD5L
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
AIM antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human AIM.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal AIM Antibody.
Purification
AIM Antibody is affinity chromatography purified via peptide column.
Host
AIM antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
AIM antibody can be used for the detection of AIM by Western blot at 1 – 4 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2 ?g/mL. For immunofluorescence start at 20 ?g/mL.
AIM Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-3807 – AIM Peptide
Storage
AIM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Miyazaki T, Hirokami Y, Matsuhashi N, et al. Increased susceptibility of thymocytes to apoptosis in mice lacking AIM, a novel murine macrophage-derived soluble factor belonging to the scavenger receptor cysteine-rich domain superfamily. J. Exp. Med. 1999; 189:413-22.
Biancone L, Bowen MA, Lim A, et al. Identification of a novel inducible cell-surface ligand of CD5 on activated lymphocytes. J. Exp. Med. 1996; 184:811-9.
Haruta I, Kato Y, Hashimoto E, et al. Association of AIM, a novel apoptosis inhibitory factor, with hepatitis via supporting macrophage survival and enhancing phagocytic function of macrophages. J. Biol. Chem.2001; 276:22910-4
Kuwata K, Watanabe H, Jiang S-Y, et al. AIM inhibits apoptosis of T cells and NKT cells in Corynebacterium-induced granuloma formation in mice. Am. J. Path. 2003; 162:837-47.]
