Akt1 Antibody: Akt1, initially identified as the cellular homolog to the retro-viral oncogene v-Akt, is part of the phosphatidyl 3′-kinase (PI3K)-Akt signaling pathway that is activated by diverse cellular stimuli and regulates critical cellular functions such as cell growth, proliferation, and survival. Following phosphorylation of the second messenger PIP2 by PI3K, Akt1 translocates to the cell membrane where it is activated by phosphoinositide-dependent kinase (PDK) 1 and PDK2. The active Akt1 is then able to phosphorylate and activate its substrates, including those that are important for cell proliferation and survival such as TOR and the Bcl-2 homolog Bad. Negative regulation of the PI3K-Akt signaling pathway is mainly accomplished by the lipid phosphatase activity of PTEN which catalyzes the conversion of PIP3 to PIP2, thereby preventing the activation of Akt1. Inactivation of this gene often results in excessive Akt1 activity, often leading to the formation of malignant tumors.
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Description
Western blot analysis of Akt1 in human liver cell lysate with Akt1 antibody at (A) 1 and (B) 2 ?g/mL.
Immunohistochemistry of Akt1 in human liver cells with Akt1 antibody at 2 ?g/mL.
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Specifications
Additional Names
Akt1 Antibody: Protein kinase B, PKB
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Isotype
IgG
Clonality/Clone
This is a polyclonal Akt1 Antibody.
Conjugate
None
Purification
Akt1 Antibody is affinity chromatography purified via peptide column.
Host
AKT1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Akt1 antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human Akt1.
Application
Akt1 antibody can be used for the detection of Akt1 by Western blot at 1 to 2 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2 ?g/mL. For immunofluorescence start at 20 ?g/mL.
Akt1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3519 – Akt1 Peptide
Storage
Akt1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Bellacosa A, Testa JR, Staal SP, et al. A retroviral oncogene, akt, encoding a serine-threonine kinase containing an SH2-like region. Science 1991; 254:274-7.
Song G, Ouyang G, and Bao S. The activation of Akt/PKB signaling pathway and cell survival. J. Cell. Mol. Med. 2005; 9:59-71.
Alessi DR, Adnjelkovic M, Caudwell B, et al. Mechanism of protein kinase B by insulin and IGF-1. EMBO J. 1996; 15:6541-51.
Nave BT, Ouwens M, Withers DJ, et al. Mammalian target of rapamycin is a direct target for protein kinase B: Identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation. Biochem. J. 1999; 344:427-31.]
