Apaf-1 Monoclonal Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
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Description
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/mL.
Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 ?g/mL.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Molecular Weight
130 kDa
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
NA
Clonality/Clone
This is a monoclonal Apaf-1 Antibody. (Clone 2E10)
Conjugate
None
Source
Mouse monoclonal Apaf1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Apaf1 (1).
Purification
Apaf-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Host
Apaf-1 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Application
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 – 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 ?g/mL.
Zou H, Henzel WJ, Liu X, et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997; 90:405-13.
Cecconi F, Alvarez-Bolado G, Meyer BI, et al. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998; 94:727-37.
Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.
Hu Y, Benedict MA, Wu D, et al. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc. Natl. Acad. Sci. USA 1998; 95:4386-91. ]
