Apaf1 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
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Description
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at 1 ?g/mL in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 ?g/mL.
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Specifications
Additional Names
Apaf1 Antibody: Apaf1, Apaf-1
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
115 – 130 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Apaf1 Antibody.
Purification
Apaf1 Antibody is affinity chromatography purified via peptide column.
Host
APAF1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
Application
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 ?g/mL. A 115 – 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 ?g/mL. For immunofluorescence start at 10 ?g/mL.
Apaf1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2013 – Apaf1 Peptide
Storage
Apaf1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299) ]
