APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
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Description
Western blot analysis of APG7 in Caco-2 cell lysate with APG7 antibody at (A) 0.5, (B) 1 and (C) 2 ?g/mL.
Immunocytochemistry of APG7 in MCF7 cells with APG7 antibody at 10 ?g/mL.
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Specifications
Additional Names
APG7 Antibody: Autophagy protein 7, ATG7
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Isotype
IgG
Clonality/Clone
This is a polyclonal APG7 Antibody.
Conjugate
None
Purification
APG7 Antibody is affinity chromatography purified via peptide column.
Host
APG7 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
APG7 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7.
Application
APG7 antibody can be used for the detection of APG7 by Western blot at 0.5 – 2 ?g/mL. Antibody can also be used for immunocytochemistry starting at 10 ?g/mL. For immunofluorescence start at 20 ?g/mL.
APG7 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3615 – APG7 Peptide
Storage
APG7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
