ARC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.
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Description
Western blot analysis of ARC in HeLa (H), KB (K), and A549 (A) whole cell lysates with ARC antibody at 1:1000 dilution.
Immunohistochemistry of ARC in human skeletal muscle with ARC antibody at 5 ?g/mL.
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Specifications
Additional Names
ARC Antibody: ARC
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
25 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal ARC Antibody.
Purification
ARC Antibody is affinity chromatography purified via peptide column.
Host
ARC antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
ARC antibody was raised against a peptide corresponding to amino acids 191 to 208 of human origin.
Application
ARC antibody can be used for detection of ARC by Western blot at 1:1000 dilution. An approximately 25 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 ?g/mL.
ARC Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2081 – ARC Peptide
Storage
ARC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-60 (RD1299)]
