ARF-BP1 Antibody: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
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Description
Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ?g/mL.
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ?g/mL.
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Specifications
Additional Names
ARF-BP1 Antibody: ARF binding protein 1; HECT, UBA and WWE domain containing 1; HUWE1; Mcl-1 ubiquitin ligase E3; Mule
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
ARF-BP1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal ARF-BP1 Antibody.
Purification
ARF-BP1 Antibody is affinity chromatography purified via peptide column.
Host
ARF-BP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 ?g/mL. Antibody can also be used for immunocytochemistry starting at 5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
ARF-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-4213 – ARF-BP1 Peptide
Storage
ARF-BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol.2006; 38:1637-41.
Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2?s inhibition of p53. Cell1998; 92:725-34.
Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell2005; 121:1071-83.
Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell2005; 121:1085-95.]
