ARMER Antibody: Apoptosis is important for normal development and tissue homeostasis. It is mediated by various caspases and ultimately results in the activation of endogenous endonucleases that degrade cellular DNA. Although apoptosis induced by endoplasmic reticulum (ER) stress is thought to be mediated by caspase-12, other caspases such as caspase-9 are also thought to be activated following ER stress. Recently, ARMER, a novel integral ER-membrane protein was shown to protect cells from ER stress-induced apoptosis. Analysis of the caspase proteolytic cascade suggests that ARMER acts by inhibiting caspase-9 activity, although the mechanism for this remains unkown. It should be noted that ARMER is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
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Description
Western blot ana-lysis of ARMER in mouse small intestine tissue lysates with ARMER antibody at (A) 0.5, (B) 1, and (C) 2 ?g/mL.
Immunohistochemical staining of human bladder tissue using ARMER antibody at 2 ?g/mL.
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Specifications
Additional Names
ARMER Antibody: ARL6IP, AIP1
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal ARMER Antibody.
Purification
ARMER Antibody is Ion exchange chromatography purified.
Host
ARMER antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
ARMER antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human ARMER.
Application
ARMER antibody can be used for detection of ARMER by Western blot at 0.5 to 2 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2 ?g/mL. For immunofluorescence start at 2 ?g/mL.
ARMER Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3305P – ARMER Peptide
Storage
ARMER antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9.
Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-?. Nature 2000; 403:98-103.
Lui HM, Chen J, Wang L, et al. ARMER, Apoptotic regulator in the membrane of the endoplasmic reticulum, a novel inhibitor of apoptosis. Mol. Cancer Res. 2003; 1:508-18.]
