ARTS Antibody: Apoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf-1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF-beta signaling pathway. ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF-beta and, to a lesser extent, by other apoptotic agents, such as TNF-? and Fas ligand.
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Description
Western blot analysis of ARTS expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with ARTS antibody at 2 ?g /ml.
Immunohistochemistry of ARTS in human lung tissue with ARTS antibody at 2 ?g/mL.
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Specifications
Additional Names
ARTS
Additional Names
ARTS Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
32 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal ARTS Antibody.
Purification
ARTS Antibody is affinity chromatography purified via peptide column.
Host
ARTS antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
ARTS antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human ARTS.
Application
ARTS antibody can be used for detection of ARTS by Western blot at 2 ?g/mL. A band at 32 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 ?g/mL. For immunofluorescence start at 20 ?g/mL.
ARTS Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3025 – ARTS Peptide
Storage
ARTS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Larisch S, Yi Y, Lotan R, Kerner H, et al. A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif. Nat Cell Biol 2000;2(12):915-21
Depraetere V. The ARTS of apoptosis. Nat Cell Biol. 2000;2(12):E219.
Paavola,P., Horelli-Kuitunen,N., Palotie,A. and Peltonen,L. Characterization of a novel gene, PNUTL2, on human chromosome 17q22-q23 and its exclusion as the Meckel syndrome gene. Genomics 1999;55 (1):122-125
Zieger,B., Tran,H., Hainmann, I. , Wunderle,D., Zgaga-Griesz,A., Blaser,S. and Ware,J. Characterization and expression analysis of two human septin genes, PNUTL1 and PNUTL2. Gene 2000;261(2):197-203 ]
