ASC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4). Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.
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Description
Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide (2287P) with ASC antibody at 1 ?g /ml.
Immunocytochemistry of ASC in HL60 cells with ASC antibody at 5 ?g/mL.
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Specifications
Additional Names
ASC
Additional Names
ASC Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
25 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal ASC Antibody.
Purification
ASC Antibody is affinity chromatography purified via peptide column.
Host
ASC antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
ASC antibody was raised against a synthetic peptide corresponding to amino acids 182 to 195 of human ASC.
Application
ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 ?g/mL. An approximately 25 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 ?g/mL.
ASC Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2287 – ASC Peptide
Storage
ASC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T, Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 1999;274(48):33835-8.
Masumoto J, Taniguchi S, Nakayama K, Ayukawa K, Sagara J. Murine Ortholog of ASC, a CARD-Containing Protein, Self-Associates and Exhibits Restricted Distribution in Developing Mouse Embryos. Exp Cell Res. 2001;262(2):128-133.
Conway KE, McConnell BB, Bowring CE, Donald CD, Warren ST , Vertino PM. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers. Cancer Res. 2000;60(22):6236-42.
McConnell BB, Vertino PM. Activation of a caspase-9-mediated apoptotic pathway by subcellular redistribution of the novel caspase recruitment domain protein TMS1. Cancer Res. 2000;60(22):6243-7. (WD0102)]
