ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-?-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.
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Description
Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 ?g/mL.
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 2 ?g/mL.
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal ASK1 Antibody.
Purification
ASK1 Antibody is affinity chromatography purified via peptide column.
Host
ASK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ASK1.
Application
ASK1 antibody can be used for the detection of ASK1 by Western blot at 0.5 – 2 ?g/mL. Antibody can also be used for immunocytochemistry starting at 2 ?g/mL. For immunofluorescence start at 20 ?g/mL.
ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3677 – ASK1 Peptide
Storage
ASK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ichijo H, Nishida E, Irie K, et al. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science1997; 275:90-4.
Wang XS, Diener K, Jannuzzi D, et al. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J. Biol. Chem.1996; 271:31607-11. ]
