ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-?-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
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Description
Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 ?g/mL.
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Specifications
Additional Names
ASK1 Antibody: MAPKKK5
Molecular Weight
155 kDa
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Isotype
IgG
Clonality/Clone
This is a polyclonal ASK1 Antibody.
Conjugate
None
Purification
ASK1 Antibody is affinity chromatography purified via peptide column.
Host
ASK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. This sequence is different from that of mouse by last two amino acids.
Application
ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 ?g/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 ?g/mL. For immunofluorescence start at 20 ?g/mL.
ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-1151 – ASK1 Peptide
Storage
ASK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T, Takagi M, Matsumoto K, Miyazono K, Gotoh Y. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 1997;275:90-4
Wang XS, Diener K, Jannuzzi D, Trollinger D, Tan TH, Lichenstein H, Zukowski M, Yao Z. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J Biol Chem 1996;271:31607-11
Tobiume K, Inage T, Takeda K, Enomoto S, Miyazono K, Ichijo H. Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1. Biochem Biophys Res Commun 1997;239:905-10 ]
