Aven Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9. It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation. Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients.
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Description
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 ?g/mL in (A) the presence and (B) the absence of blocking peptide.
Immunohistochemistry of Aven in human spleen tissue with Aven antibody at 5 ?g/mL.
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
Aven antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Aven.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Aven Antibody.
Purification
Aven Antibody is affinity chromatography purified via peptide column.
Host
Aven antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Aven antibody can be used for detection of Aven by Western blot at 1 ?g/mL. Despite its predicted molecular weight, Aven often migrates at 55 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
Aven Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-2417 – Aven Peptide
Storage
Aven antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
