BAG-1 Antibody: Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.
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Description
Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 ?g/mL.
Immunohistochemistry of BAG-1 in human lymph node tissue with BAG-1 antibody at 2 ?g/mL
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Specifications
Additional Names
BAG-1 Antibody: Bcl-2-binding athanogene-1, receptor-associated protein 46, RAP46
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
BAG-1 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human BAG-1.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal BAG-1 Antibody.
Purification
BAG-1 Antibody is affinity chromatography purified via peptide column.
Host
BAG-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
BAG-1 antibody can be used for the detection of BAG-1 by Western blot at 1 – 2 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2 ?g/mL. For immunofluorescence start at 20 ?g/mL.
BAG-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-3871 – BAG-1 Peptide
Storage
BAG-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Takayama S, Sato T, Kraweski K, et al. Cloning and functional analysis of BAG-1: a novel Bcl2-binding protein with anti-cell death activity. Cell 1995; 80:279-84.
Nollen EAA, Brunsting JF, Song J, et al. Bag1 functions in vivo as a negative regulator of Hsp70 chaperone activity. Mol. Cell. Biol. 2000; 20:1083-8.
Cato ACB and Mink S. BAG-1 family of cochaperones in the modulation of nuclear receptor action. J. Steroid Biochem. & Mol. Biol. 2001; 78:379-88.
Kajewska M, Turner BC, Shabaik A, et al. Expression of BAG-1 protein correlates with aggressive behavior of prostate cancers. Prostate 2006; 66:801-10.]
