Bak Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bak, a pro-apoptotic Bcl-2 family member, is an oligomeric protein that localizes to the mitochondria. It is thought to share significant functional homology with Bax, another pro-apoptotic Bcl-2 family member, as disruption of bak or bax has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking bak and bax show decreased apoptotic capability.
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Description
Western blot analysis of Bak in L1210 cell lysates with Bak antibody at (A) 1 and (B) 2 ?g/mL.
Immunocytochemistry of Bak in L1210 cells with Bak antibody at 2 ?g/mL.
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Specifications
Additional Names
Bak (NT), Bak
Additional Names
Bak Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bak Antibody.
Purification
Bak Antibody is Ion exchange chromatography purified.
Host
Bak antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Bak antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of human Bak.
Application
Bak antibody can be used for detection of Bak by Western blot at 1 to 2 ?g/mL. Antibody can also be used for immunocytochemistry starting at 2 ?g/mL.
Bak Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3347 – Bak Peptide
Storage
Bak antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
