Bcl-10 Antibody: Apoptosis is related to many diseases including cancer. Cell death signals are transduced by death domain (DD) and caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include ARC, RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD containing protein was recently identified by several groups and designated Bcl10, CIPER, mE10, CARMEN, CLAP. Bcl10 is a cellular homolog of the equine herpesvirus-2 E-10 gene. Overexpression of Bcl10 induces JNK, p38, and NF-?B activation. Bcl10 interacts with caspase-9 and enhances pro-caspase-9 processing and induces apoptosis through caspase-9 activation. Bcl10 exhibits a variety of mutations in MALT lymphomas and in B and T cell lineage lymphomas indicating that it may be commonly involved in the pathogenesis of human malignancy. Bcl10 is expressed in many human and murine tissues and cell lines.
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Description
Western blot analysis of Bcl-10 in Raji whole cell lysate in the absence (A) or presence (B) of peptide (2161P) with Bcl-10 antibody at 1:500 dilution.
Immunocytochemistry of Bcl10 in Raji cells with Bcl10 antibody at 1 ?g/mL.
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Specifications
Additional Names
Bcl-10 Antibody: CIPER, CLAP
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
31 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bcl-10 Antibody.
Purification
Bcl-10 Antibody is affinity chromatography purified via peptide column.
Host
Bcl-10 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Bcl10 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Bcl10.
Application
Bcl-10 antibody can be used for detection of BCL10 by Western blot at 0.5 ?g/mL. dilution. An approximately 31 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 1 ?g/mL. For immunofluorescence start at 10 ?g/mL.
Bcl-10 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2161 – Bcl-10 Peptide
Storage
Bcl-10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Willis TG, Jadayel DM, Du MQ, et al. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types. Cell 1999;96(1):35-45
Koseki T, Inohara N, Chen S, et al. CIPER, a novel NF ?B-activating protein containing a caspase recruitment domain with homology to Herpesvirus-2 protein E10. J Biol Chem 1999;274(15):9955-61
Yan M, Lee J, Schilbach S, Goddard A, Dixit V. mE10, a novel caspase recruitment domain-containing proapoptotic molecule. J Biol Chem 1999;274(15):10287-92
Thome M, Martinon F, Hofmann K, et al. Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-?B transcription factor and c-Jun N-terminal kinase. J Biol Chem 1999;274(15):9962-8 ]
