Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
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Description
Western blot analysis of Bcl-2 in Daudi cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 ?g/mL.
Immunohistochemical staining of human kidney using Bcl-2 antibody at 2 ?g/mL.
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Specifications
Additional Names
Bcl-2 (NT), Bcl-2
Additional Names
Bcl-2 Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bcl-2 Antibody.
Purification
Bcl-2 Antibody is Ion exchange chromatography purified.
Host
Bcl-2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Bcl-2 antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2.
Application
Bcl-2 antibody can be used for detection of Bcl-2 by Western blot at 1 to 4 ?g/mL. Bcl-2 antibody can also detect Bcl-2 by immunohistochemistry at 2 ?g/mL. For immunofluorescence start at 10 ?g/mL.
Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3335 – Bcl-2 Peptide
Storage
Bcl-2antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
