Bcl-B Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1.
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Description
Western blot analysis of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 ?g/ml in the (A) absence and (B) presence of blocking peptide.
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 ?g/mL.
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Specifications
Additional Names
Bcl-B Antibody: Boo, Diva, Nrh
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
Bcl-B antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bcl-B Antibody.
Purification
Bcl-B Antibody is affinity chromatography purified via peptide column.
Host
Bcl-B antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 ?g/mL. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications. Antibody can also be used for immunocytochemistry starting at 10 ?g/mL.
Bcl-B Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-3163 – Bcl-B Peptide
Storage
Bcl-B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
