Bcl-G Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.
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Description
Western blot analysis of Bcl-G in U937 cell lysates with Bcl-G antibody at (A) 2.5 and (B) 5 ?g/mL.
Immunohistochemical staining of mouse testis tissue using Bcl-G antibody at 2 ?g/mL.
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Specifications
Additional Names
Bcl-G Antibody: Bcl-2-like 14
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Specificity
Although antibody should react with both isoforms, only the Bcl-GS protein has been observed
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bcl-G Antibody.
Purification
Bcl-G Antibody is Ion exchange chromatography purified.
Host
Bcl-G antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Bcl-G antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bcl-G.
Application
Bcl-G antibody can be used for detection of Bcl-G by Western blot at 2.5 to 5 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2 ?g/mL. For immunofluorescence start at 10 ?g/mL.
Bcl-G Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3165 – Bcl-G Peptide
Storage
Bcl-G antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
