Bcl-rambo Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.
?
Description
Western blot analysis of Bcl-rambo in K562 cell lysate with Bcl-rambo antibody at (A) 2 and (B) 4 ?g/mL.
Immunohistochemistry of Bcl-rambo in human heart tissue with Bcl-rambo antibody at 10 ?g/mL.
?
?
?
?
?
?
?
?
?
?
Specifications
Additional Names
Bcl-rambo Antibody: Bcl-2 like protein 13, Mil1
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Bcl-rambo Antibody.
Purification
Bcl-rambo Antibody is affinity chromatography purified via peptide column.
Host
Bcl-rambo antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Bcl-rambo antibody was raised against a 15 amino acid synthetic peptide from near the center of human Bcl-rambo.
Application
Bcl-rambo antibody can be used for the detection of Bcl-rambo by Western blot at 2 and 4 ?g/mL. Antibody can also be used for immunohistochemistry starting at 10 ?g/mL. For immunofluorescence start at 20 ?g/mL.
Bcl-rambo Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3023 – Bcl-rambo Peptide
Storage
Bcl-rambo antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
Kataoka T, Holler N, Michau O, et al. Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension. J. Biol. Chem. 2001; 276:19548-54.,]
