FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.
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Description
Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1:1000 dilution.
Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 10 ?g/mL.
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Specifications
Additional Names
FLIP Antibody: I-FLICE
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
55 kDa
Specificity
Antibody recognizes the FLIPa only.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal FLIP Antibody.
Purification
FLIP Antibody is Antibody is DEAE purified.
Host
FLIP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
FLIP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human FLIPaFLIPl form.
Application
Flip antibody can be used for detection of FLIP by Western blot at 1 ?g/mL. A 55 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 ?g/mL. For immunofluorescence start at 10 ?g/mL.
FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-1161 – FLIP Peptide
Storage
FLIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Thome M, Schneider P, Hofmann K, et al. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521
Irmler M, Thome M, Hahne M, et al. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195
Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763
Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257]
