Hax1a Monoclonal Antibody: The HS-1 associated protein X-1 (Hax1) was initially identified in a yeast two-hybrid assay on the basis of its ability to bind to the hemapoietic cell-specific protein 1 (HS-1). Hax1 possesses anti-apoptotic activity and is structurally related to Bcl-2 family members, including the presence of BH1- and BH2-like domains. It has recently been shown to interact with HIV viral protein R (Vpr), a protein required for viral pathogenesis of HIV and linked to T-cell apoptosis through activation of caspases 3 and 9. Other studies indicate that Hax1-mediated processing of HtrA2 (also known as Omi) by the mitochondrial protease PARL allows survival of lymphocytes and neurons when cytokines are limiting. At least four isoforms of Hax1 are known to exist. This antibody is expected to recognize the longest isoform (Hax1a) as well as the shortest.
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Description
Western blot analysis of Hax1a in human brain tissue lysate with Hax1a antibody at (A) 1 and (B) 2 ?g/mL.
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Specifications
Additional Names
Hax1a Monoclonal Antibody: Hax1a (8F9G7), HS-1 associated protein X-1, HCLS1 associated protein X-1, HCLSBP1, HS1BP1
User Note
Optimal dilutions for each application to be determined by the researcher.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Isotype
NA
Conjugate
None
Clonality/Clone
This is a monoclonal Hax1a Antibody. (Clone 8F9G7)
Source
Mouse monoclonal Hax1a antibody was raised against a 15 amino acid peptide near the amino terminus of human Hax1a.
Purification
Hax1a Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Host
Hax1a monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Mouse monoclonal Hax1a antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human Hax1a.
Application
Hax1a antibody can be used for detection of Hax1A by Western blot at 1 – 2 ?g/mL.
Suzuki Y, Demoliere C, Kitamura D, et al. HAX-1, a novel intracellular protein, localized on mitochondria directly associates with HS1, a substrate of Src family tyrosine kinases. J. Immunol. 1997; 158:2736-44.
Sharp TV, Wang HW, Koumi A, et al. K15 protein of Kaposi?s sarcoma-associated herpesvirus is latently expressed and binds to HAX-1, a protein with antiapoptotic function. J. Virol. 2002; 76:802-16.
Yedavalli VS, Shih HM, Chiang YP, et al. Human immunodeficiency virus type 1 Vpr interacts with antiapoptotic mitochondrial protein HAX-1. J. Virol. 2005; 79:13735-46.
Chao J-R, Parganas E, Boyd K, et al. Hax1-mediated processing of HtrA2 by Parl allows survival of lymphocytes and neurons. Nature 2008; 452:98-102.]
