NALP1 Antibody: NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.
?
Description
Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 ?g/mL.
Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 ?g/mL.
?
?
?
?
?
?
?
?
Specifications
Additional Names
NALP1 Antibody: CARD7, DEFCAP, NACHT domain- leucine-rich-repeat and PYD-containing protein 1
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal NALP1 Antibody.
Purification
NALP1 Antibody is immunoaffinity chromatography purified IgG.
Host
NALP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1.
Application
NALP1 antibody can be used for detection of NALP1 by Western blot at 1 to 4 ?g/mL. Antibody can also be used for immunocytochemistry starting at 10 ?g/mL. For immunofluorescence start at 20 ?g/mL.
NALP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3037 – NALP1 Peptide
Storage
NALP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
NALP1 antibody can be stored at 4˚C, stable for one year. Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104.
Chu ZL, Pio F, Xie Z, et al. A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis. J. Biol. Chem. 2001; 276:9239-45.
Hlaing T, Guo RF, Dilley KA, et al. Molecular cloning and characterization of DEFCAP-L and -S, two isoforms of a novel member of the Ced-4 family of apoptosis proteins. J. Biol. Chem. 2001; 276:9230-8.
Sanz C, Calasanz MJ, Andreu E, et al. NALP1 is a transcriptional target for cAMP-response-element-binding protein (CREB) in myeloid leukemia cells. Biochem. J. 2004; 384:281-6.]
