NALP3 Antibody: NALP3, a member of Nod-like receptors, has a crucial role in inflammation and immunity and may be a proximal sensor of cellular stress and danger signals. NALP3 forms a caspase-1 activating molecular complex termed the inflammasome. The inflammasome allows activation of IL-1?, the key player of the inflammation and fever. NALP3 gene encodes a pyrin like protein which contains a pyrin domain, a nucleotide binding site (NBS) domain, and a leucine rich repeat (LRR) motif. NALP3 protein interacts with apoptosis associated speck like protein containing a CARD and may function as an inducer of apoptosis and an activator of NF-?B signaling. Defects in NALP3 have been associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).
?
Description
Western blot analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ?g/mL (A) in the absence and (B) in the presence of blocking peptide.
Immunofluorescence of NALP3 in K562 cells with NALP3 antibody at 20 ?g/mL.
?
?
?
?
?
?
?
?
?
?
?
?
?
Specifications
Additional Names
NALP3 Antibody: LRR and PYD domains-containing protein 3, CIAS1, FCAS, Cryopyrin
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
NALP3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human NALP3.
Isotype
IgG
Clonality/Clone
This is a polyclonal NALP3 Antibody.
Conjugate
None
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Purification
NALP3 Antibody is affinity chromatography purified via peptide column.
Host
NALP3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
NALP3 antibody can be used for detection of NALP3 by Western blot at 1 ?g/mL. Antibody can also be used for immunoflourescence starting at 20 ?g/mL. For immunofluorescence start at 20 ?g/mL.
NALP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-5447 – NALP3 Peptide
Storage
NALP3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Chamaillard M, Girardin SE, Viala J, et al. Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation. Cell. Microbiol.2003; 5:581-92.
O’Connor W Jr., Hartin JA, Zhu X, et al. Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties. J. Immunol.2003; 171:6329-33.
Kanneganti TD, Ozoren N, Body-Malapal M, et al. Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp. Nature2006; 440:233-6.
Agostini L, Martinon F, Burns K, et al. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 2004; 20:319-25.]
