PDCD4 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.
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Description
Western blot analysis of PDCD4 in A-20 cell lysate with PDCD4 antibody at (A) 0.5 and (B) 1?g/mL.
Immunohistochemistry of PDCD4 in mouse brain tissue with PDCD antibody at 2.5 ?g/mL.
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Specifications
Additional Names
PDCD4 Antibody: Programmed cell death 4, neoplastic transformation inhibitor
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
PDCD4 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human PDCD4.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal PDCD4 Antibody.
Purification
PDCD4 Antibody is affinity chromatography purified via peptide column.
Host
PDCD4 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
PDCD4 antibody can be used for detection of PDCD4 by Western blot at 0.5 – 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ?g/mL.
PDCD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-3975 – PDCD4 Peptide
Storage
PDCD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63.
Shibihara K, Asano M, Ishida Y, et al. Isolation of a novel mouse gene MA-3 that is induced upon programmed cell death. Gene 1995; 166:297-301.
Goke R, Barth P, Schmidt A, et al. Programmed cell death 4 suppresses CDK1/cdc2 via induction of p21 (Waf1/Cip1). Am. J. Physiol. Cell Physiol. 2004; 287:C1541-6.
Yang HS, Jansen AP, Nair R, et al. The transformation suppressor Pdcd4 is a novel translation initiation factor 4A binding protein that inhibits translation. Mol. Cell Biol. 2003; 23:26-37.]
