PDL-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. PDL-1 antibody has no cross-reactivity to PDL-2.
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Description
Western blot analysis of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 ?g/mL.
Immunohistochemistry of PDL-1 in human heart tissue with PDL-1 antibody at 2.5 ?g/mL.
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Specifications
Additional Names
PDL-1 Antibody: PDL-1, Programmed cell death 1 ligand-1, programmed death ligand 1, PD-L1, B7-H1
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
PDL-1 antibody was raised against a 17 amino acid synthetic peptide from near the center of human PDL-1.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal PDL-1 Antibody.
Purification
PDL-1 Antibody is affinity chromatography purified via peptide column.
Host
PDL-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
PDL-1 antibody can be used for detection of PDL-1 by Western blot at 0.5 – 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
PDL-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-4059 – PDL-1 Peptide
Storage
PDL-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.]
