PHAP Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
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Description
Western blot analysis of PHAP expression in human Raji cell lysate with PHAP antibody at 1 ?g/mL. The wide and strong band below PHAP III is a doublelet composed of PHAP I (upper) and PHAP I2a (lower).
Immunocytochemistry of PHAP in Raji cells with PHAP antibody at 1 ?g/mL.
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Specifications
Additional Names
PHAP (NT), PHAP
Additional Names
PHAP Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal PHAP Antibody.
Purification
PHAP Antibody is affinity chromatography purified via peptide column.
Host
PHAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
PHAP antibody was raised with a synthetic peptide corresponding to amino acids at amino terminus of human PHAP.
Application
PHAP antibody can be used for detection of all three identified isoforms of PHAP including PHAP I, PHAP I2a, and PHAP III by Western blot at 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 1 ?g/mL.
PHAP Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-3149 – PHAP Peptide
Storage
PHAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-? in a death regulatory pathway. Science. 2003;299(5604):223-6.
Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299(5604):214-5.]
