PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA? and PUMA?. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Description
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 ?g/mL.
Optimal dilutions for each application to be determined by the researcher.
Disclaimer
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Isotype
IgG1
Clonality/Clone
This is a monoclonal PUMA Antibody. (Clone 2A9G5)
Conjugate
None
Source
Mouse monoclonal PUMA antibody was raised against a recombinant protein corresponding to amino acids 76 – 170 of human PUMA-alpha.
Purification
PUMA Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Host
PUMA monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Recombinant protein corresponding to amino acids 76 – 170 of human PUMA-alpha.
Application
PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 ?g/ml.
Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.]
