RAIDD Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1, RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1, 3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.
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Description
Western blot analysis of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution.
Immunocytochemistry of RAIDD in HeLa cells with RAIDD antibody at 5 ?g/mL.
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Specifications
Additional Names
RAIDD Antibody: CRADD
Molecular Weight
22 kDa
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Isotype
IgG
Clonality/Clone
This is a polyclonal RAIDD Antibody.
Conjugate
None
Purification
RAIDD Antibody is affinity chromatography purified via peptide column.
Host
RAIDD antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
RAIDD antibody was raised against a peptide corresponding to amino acids 99 to 117 of human RAIDD.
Application
RAIDD antibody can be used for detection of RAIDD by Western blot at 0.5 – 1 ?g/mL. A 22 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
RAIDD Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-1115 – RAIDD Peptide
Storage
RAIDD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Duan H, Dixit VM. RAIDD is a new ‘death’ adaptor molecule. Nature 1997;385:86-89
Ahmad M, Srinivasula SM, Wang L, Talanian RV, Litwack G, Fernandes-Alnemri T, Alnemri ES. CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor receptor-interacting protein RIP. Cancer Res 1997 57:615-619
Hofmann K, Bucher P, Tschopp J. The CARD domain: a new apoptotic signalling motif. Trends Biochem Sci 1997;22:155-156 (RD1299) ]
