SODD Antibody: Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
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Description
Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide (Catalog no. 2143P) with SODD antibody at 1:500 dilution.
Immunocytochemistry of SODD in HeLa cells with SODD antibody at 5 ?g/mL.
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Specifications
Additional Names
SODD (NT), SODD
Additional Names
SODD Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
60 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal SODD Antibody.
Purification
SODD Antibody is Antibody is DEAE purified.
Host
SODD antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
SODD antibody was raised against a peptide corresponding to amino acids near the amino terminus of human SODD.
Application
SODD antibody can be used for detection of SODD by Western blot at 0.5 ?g/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 ?g/mL. For immunofluorescence start at 20 ?g/mL.
SODD Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2143 – SODD Peptide
Storage
SODD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Jiang Y, Woronicz JD, Liu W, Goeddel DY. Prevention of constitutive TNF receptor 1 signaling by silencer of death domains. Science 1999;283:543-6 (RD1299)]
