Survivin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.
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Description
Western blot analysis of survivin in MOLT4 cell lysate in the absence (A) or presence (B) of blocking peptide with survivin antibody at 1 ?g/mL.
Immunocytochemistry staining of MOLT4 cells using survivin at 10 ?g/mL.
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Specifications
Additional Names
Survivin Antibody: TIAP
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
17 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Survivin Antibody.
Purification
Survivin Antibody is affinity chromatography purified via peptide column.
Host
Survivin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Survivin antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human survivin.
Application
Survivin antibody can be used for detection of survivin by Western blot at 1 ?g/mL. An approximate 17 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 ?g/mL. For immunofluorescence start at 10 ?g/mL.
Survivin Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2235 – Survivin Peptide
Storage
Survivin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 1997;3:917-21
Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC. Control of apoptosis and mitotic spindle checkpoint by survivin. Nature 1998;396:580-4
Kobayashi K, Hatano M, Otaki M, Ogasawara T, Tokuhisa T. Expression of a murine homologue of the inhibitor of apoptosis protein is related to cell proliferation. Proc Natl Acad Sci USA 1999;96:1457-62 (WD0600) ]
