TACE Antibody: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-? is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-?. TNF-? converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
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Description
Western blot analysis of TACE in HeLa and Jurkat whole cell lysate in the absence or presence of blocking peptide (Cat. No. 1131P) with TACE antibody at 1:500 dilution.
Immunocytochemistry of TACE in HeLa cells with TACE antibody at 10 ?g/mL.
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Specifications
Additional Names
TACE Antibody: adam17
Molecular Weight
Predicted: 93 kDa
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Specificity
80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE.
Isotype
IgG
Clonality/Clone
This is a polyclonal TACE Antibody.
Conjugate
None
Purification
TACE Antibody is affinity chromatography purified via peptide column.
Host
TACE antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
TACE antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human TACE. This sequence differs from those of mouse and rat TACE by one amino acid.
Application
TACE antibody can be used for detection of TACE by Western blot at 0.5 ?g/mL. For immunocytochemistry use 10 ?g/mL. For immunofluorescence start at 10 ?g/mL.
TACE Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-1131 – TACE Peptide
Storage
TACE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Black RA, Rauch CT , Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-? from cells. Nature 1997;385:729-733
Moss ML, Jin SL, Milla ME, et al. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-?. Nature 1997;385:733-736
Mizui Y, Yamazaki K, Sagane K, Tanaka I. cDNA cloning of mouse tumor necrosis factor-? converting enzyme (TACE) and partial analysis of its promoter. Gene 1999;233:67-74
Peschon JJ, Slack JL, Reddy P, et al. An essential role for ectodomain shedding in mammalian development. Science 1998;282:1281-4]
