TCCR Antibody: Upon antigen challenge, T-helper cells differentiate into two functional distinct subsets, Th1 and Th2. Th1 cells produce IL-2, IFN-gamma and lymphotoxin-beta that augment cell mediated immune response while Th2 cells secrete IL-4, IL-5, and IL-10 that enhance humoral immunity. The function of T-helper cells is regulated by cytokines. A novel cytokine receptor was recently identified and cloned. It is a new member in the type I cytokine receptor family and designated TCCR for T-cell cytokine receptor and WSX-1. TCCR deficient mice had impaired Th1 responses to protein antigen challenge, including decreased levels of IFN-gamma and Th1-dependent antibody IgG2a. TCCR is predominately expressed in thymus, spleen, lymph notes and peripheral blood leukocytes.
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Description
Western blot analysis of TCCR expression in human spleen tissue lysates with TCCR antibody at 1 ?g /ml.
Immunohistochemistry of TCCR in human spleen tissue with TCCR antibody at 10 ?g/mL.
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Specifications
Additional Names
TCCR Antibody: Type I T-cell cytokine receptor, IL-27 receptor alpha, IL-27Ra
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
70 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal TCCR Antibody.
Purification
TCCR Antibody is affinity chromatography purified via peptide column.
Host
TCCR antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
TCCR antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human TCCR precursor.
Application
TCCR antibody can be used for detection of TCCR by Western blot at 1 ?g/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 10 ?g/mL. For immunofluorescence start at 20 ?g/mL.
TCCR Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. ZP-2481 – TCCR Peptide
Storage
TCCR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Chen Q, Ghilardi N, Wang H, Baker T, Xie MH, Gurney A, Grewal IS and de Sauvage FJ. Development of Th1-type immune responses requires the type I cytokine receptor TCCR Nature 2000;407(6806):916-920
Sprecher,C.A., Grant,F.J., Baumgartner,J.W., Presnell,S.R., Schrader,S.K., Yamagiwa,T., Whitmore,T.E., O’Hara,P.J. and Foster,D.F. Cloning and characterization of a novel class I cytokine receptor Biochem. Biophys. Res. Commun. 1998;246(1):82-90 ]
