TIGAR Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death; loss of its functions contributes to the development of most cancers. Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase. TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress. TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.
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Description
Western blot analysis of TIGAR in EL4 cell lysate with TIGAR antibody at (A) 0.5, (B) 1 and (C) 2 ?g/mL.
Immunohistochemistry of TIGAR in human brain tissue with TIGAR antibody at 2.5 ?g/mL.
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Specifications
Additional Names
TIGAR Antibody: Tp53-induced glycolysis and apoptosis regulator
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
TIGAR antibody was raised against a 18 amino acid synthetic peptide from near the center of human TIGAR.
Isotype
IgG
Clonality/Clone
This is a polyclonal TIGAR Antibody.
Conjugate
None
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Purification
TIGAR Antibody is affinity chromatography purified via peptide column.
Host
TIGAR antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
TIGAR antibody can be used for detection of TIGAR by Western blot at 0.5 – 2 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ?g/mL.
TIGAR Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-4049 – TIGAR Peptide
Storage
TIGAR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
