Toso Antibody: Apoptosis is an important process by which normal tissue homeostasis and function are maintained. One of the major signals that regulate this process is mediated by the activation of the Fas receptor by its ligand. This leads to the formation of a Fas-associated death domain (FADD)- containing death-inducing signaling complex and the activation of caspase-8, which in turn activates downstream effector caspases, such as caspase-3 and -7. Recent experiments have shown that overexpression of Toso, a novel regulator of Fas-induced apoptosis in lymphoid cells, in Jurkat cells as well as transgenic mice render these cells resistant to Fas-induced apoptosis but not to TRAIL-induced apoptosis. Furthermore, Toso was found to associate with FADD, suggesting that Toso functions by disrupting the formation of the death-inducing signaling complex.
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Description
Western blot analysis of Toso in human lung tissue lysate with Toso antibody at 1 ?g/mL in either the (A) presence, or (B) absence of blocking peptide.
Optimal dilutions for each application to be determined by the researcher.
Immunogen
Toso antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Toso.
Isotype
IgG
Clonality/Clone
This is a polyclonal Toso Antibody.
Conjugate
None
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Purification
Toso Antibody is affinity chromatography purified via peptide column.
Host
Toso antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Toso antibody can be used for detection of Toso by Western blot at 1 ?g/mL. Despite its predicted molecular weight, Toso often migrates at 60 kDa in SDS-PAGE.
Toso Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-2273 – Toso Peptide
Storage
Toso antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Curtin JF and Cotter TG. Live and let die: regulatory mechanisms in Fas-mediated apoptosis. Cell Signal. 2003; 15:983-92.
Hitoshi Y, Lorens J, Kitada S-I, et al. Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells. Immunity 1998; 8:461-71.
Song Y and Jacob CO. The mouse cell surface protein Toso regulates Fas/Fas ligand-induced apoptosis through its binding to Fas-associated death domain. J. Biol. Chem.2005; 280:9618-26.]
