TWEAK Antibody: TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane. TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, in addition to stimulation of apoptosis. The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains.
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Description
Western blot analysis of (A) 5 ng, (B) 25 ng and (C) 50 ng of recombinant TWEAK with TWEAK antibody at 1 ?g/mL.
Immunohistochemistry of TWEAK in human brain tissue with TWEAK antibody at 10 ?g/mL.
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Specifications
Additional Names
TWEAK Antibody: TNF-related weak inducer of apoptosis, TNF ligand superfamily member 12
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid?
Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal TWEAK Antibody.
Purification
TWEAK Antibody is Purified IgG.
Host
TWEAK antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
TWEAK antibody was raised against recombinant human TWEAK.
Application
TWEAK antibody can be used for the detection of TWEAK by Western blot at 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 10 ?g/mL.
TWEAK Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. ZP-3791 – TWEAK Peptide
Storage
TWEAK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Wiley SR and Winkles JA. TWEAK, a member of the TNF superfamily, is a multifunctional cytokine that binds the TweakR/Fn14 receptor. Cyto. And Growth Factor Rev. 2003; 14:241-9.
Chicheportiche Y, Bourdon PR, Xu H, et al. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J. Biol. Chem. 1997; 272:32401-10.
Lynch CN, Wang YC, Lund JK, et al. TWEAK induces angiogenesis and proliferation of endothelial cells. J. Biol. Chem. 1999; 274:8455-9.
Chicheportiche Y, Chicheportiche R, Sizing I, et al. Proinflammatory activity of TWEAK on human dermal fibroblasts and synoviocytes: blocking and enhancing effects of anti-TWEAK monoclonal antibodies. Arthr. Res. 2002; 4:126-33. 5.]
