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Zyagen offers the largest selection of
Northern blots available for mouse, rat, human, dog, hamster, Guinea pig, and
rabbit tissues to fit the specific need of
scientists from different research fields. Blots are prepared from high quality
total RNA blotted onto positively charged nylon or nitrocellulose membranes.
Blots can be used with either
radioactive or nonisotopic-labeled probes to:
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Quickly
assess gene expression across rat or mouse tissues.
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Determine size and relative abundance
of transcripts in different tissues.
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Identify alternative splice forms.
Benefits:
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Ready-to-hybridize Northern blots from total RNA
of mouse, rat, human, dog, hamster, Guinea pig, and rabbit tissues that are difficult to obtain because of
size, high RNase content, size, and anatomical complexity which
requires knowledge, experience, and technical skill to exactly dissect the area
of interest freely of surrounding tissues.
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Saving labor by eliminating tedious and
time-consuming laboratory procedures (harvesting and homogenization of tissues,
RNA extraction, RNA quantification, gel electrophoresis, and blotting).
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High standards of quality control
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Economic, using multiple tissues at a cost much
lower than it would take to do in your laboratory. Think to the labor and cost
of laboratory materials, supplies and animals (purchasing, transportation,
housing, disposable of carcasses and waste materials).
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Reusable for several times, just strip probe and
re-hybridize with new one.
Quality control:
Every step of preparation of blots, from
harvesting tissues and extraction of RNA to the blotting, is carefully
monitored to ensure the superior quality and performance.
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Blots are made from RNA that is treated with RNase-free DNase to remove
residual DNA. The purity and
integrity of RNA are tested by denaturing agarose gel electrophoresis.
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The efficiency of transfer is checked by staining gels with reversible staining .
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The integrity of the blotted RNA is tested by beta-actin specific probe.
Description:
The freshly isolated total RNA samples (20mg
each) of mouse, rat, human, dog, hamster, Guinea pig, and rabbit
tissues
are fractionated though
formaldehyde-denaturing agarose gel eletrophoresis,
blotted onto a positively charged nylon membranes, and immobilized by UV light.
Each blot contains up to 22 samples in addition to the RNA molecular size
standard. Blot is shipped in a sealed bag at room temperature and is good for 1 year if stored at 4oC in
the sealed bags.
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