|
Human, dog, hamster, guinea pig, and rabbit tissue Northern blots are made from high
quality total RNA,
which is subjected to rigorous quality control procedures and blotted onto
positively charged nylon or nitrocellulose membranes.
Blots can be used with either
radioactive or nonisotopic-labeled probes to:
-
Quickly
assess gene expression across different tissues.
-
Determine size and relative abundance
of transcripts in the tissues.
-
Identify alternative splice forms.
Quality control:
Every step of preparation of blots, from
harvesting tissues and extraction of RNA to the blotting, is carefully
monitored to ensure the superior quality and performance.
-
Blots are made from RNA that is treated with RNase-free DNase to remove
residual DNA. The purity and
integrity of RNA are tested by denaturing agarose gel electrophoresis.
-
The efficiency of transfer is checked by staining gels with reversible staining .
-
The integrity of the blotted RNA is tested by beta-actin specific probe.
Back to the Northern blot
main page for more technical information.
Custom Services: Custom Services: If you are interested in Western blot
not listed below or required blot to be specifically prepared to meet
your application, our custom service scientists can make it for you. We can also
make ready to use Western blots of bovine, sheep, porcine, equine, and chicken
mixed tissues.
Click
here
for more information or contact our technical service representative at
zinfo@zyagen.com or at 858 546 0720.
|
